CDC6 (phospho S54) polyclonal antibody
产品名称： CDC6 (phospho S54) polyclonal antibody
英文名称： CDC6 (phospho S54) polyclonal antibody
产品编号： PAB0435
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC6.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S54 of human CDC6.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Cdc6 chromatin affinity is unaffected by serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A.
Alexandrow MG, Hamlin JL.Mol Cell Biol. 2004 Feb;24(4):1614-27.
- 2.
- Caspase-3-mediated cleavage of Cdc6 induces nuclear localization of p49-truncated Cdc6 and apoptosis.
Yim H, Jin YH, Park BD, Choi HJ, Lee SK.Mol Biol Cell. 2003 Oct;14(10):4250-9. Epub 2003 Jun 27.
- 3.
- Human replication protein Cdc6 prevents mitosis through a checkpoint mechanism that implicates Chk1.
Clay-Farrace L, Pelizon C, Santamaria D, Pines J, Laskey RA.EMBO J. 2003 Feb 3;22(3):704-12.
- Applications
- Western Blot (Tissue lysate)
- CDC6 (phospho S54) polyclonal antibody (Cat # PAB0435). Western blot analysis of CDC6 expression in human colon.
- Protocol Download
- Western Blot (Cell lysate)
- CDC6 (phospho S54) polyclonal antibody (Cat # PAB0435). Western blot analysis of CDC6 expression in HeLa.
- Protocol Download
- Western Blot (Cell lysate)
- The CDC6 (phospho S54) polyclonal antibody (Cat # PAB0435) is used in Western blot to detect Phospho-CDC6-S54 in Ramos cell lysate
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC6 (phospho S54) polyclonal antibody (Cat # PAB0435) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 990
- Protein Accession#:
- NP_001245;Q99741
- Gene Name:
- CDC6
- Gene Alias:
- CDC18L,HsCDC18,HsCDC6
- Gene Description:
- cell division cycle 6 homolog (S. cerevisiae)
- Omim ID:
- 602627
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq
- Other Designations:
- CDC18 (cell division cycle 18, S.pombe, homolog)-like,CDC6 cell division cycle 6 homolog,CDC6-related protein,cell division cycle 6 protein
- Interactome
- Gene Pathway