Product: Anti-Bcl-rambo (NT), clone Rocky-1
Cat. No.: MC-408 (100 μg)
Synonyms:
Bcl-2-like Protein 13, Mil1 Protein.
Specificity:
Recognizes the N-terminus of human Bcl-rambo
(aa 1-223). Detects endogenous proteins by ICC,
IP and WB.
Species Reactivity:
Human. Other species not tested.
Ig Isotype:
Mouse IgG1
Immunogen:
Recombinant human Bcl-rambo fused at the Nterminus
to a tag.
Format:
100 μL of antibody purified from concentrated
hybridoma tissue culture supernatant. Antibody
is at 1 mg/mL in PBS with 0.02% sodium azide.
Purity:
>95% by SDS-PAGE analysis.
Storage:
Store at 4°C short term and at -20°C long term.
Aliquot to avoid freeze/thaw cycles.
Applications and Suggested Dilutions:
􀂄 Western blot: Use at a 1:1,000 dilution.
􀂄 Immunoprecipitation: Use at a 1:100 dilution.
􀂄 Immunocytochemistry: Use at a 1:300 dilution.
The optimal dilution for a specific application
should be determined by the researcher.
Figure: Immunofluorescence microscopy showing the
detection of endogenous Bcl-rambo in HELA cells with the
antibody to Bcl-rambo (human) (NT) (MC-408)
Method: Following fixation with 2% paraformaldehyde/PBS,
HELA cells were incubated with the antibody to Bcl-rambo
(human) (NT) (MC-408) at a 1:300 dilution. An anti-mouse
IgG antibody conjugated with Alexa Fluor 488 was used for
detection.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.