Synonym: EGF 
CAS Number: 62253-63-8 
MDL number: MFCD00132565 
Description
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Analysis Note ≥98% SDS-PAGE 
Biochem/physiol Actions Qualified for use as a mitogen for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells. 
Physical form Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline 
Application Qualified for use as a mitogen for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells. 
General description Epidermal Growth Factor (EGF) is a small mitogenic polypeptide which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids. Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion. EGF is a member of a growth factor family which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The location of 3 intrachain disulfide bonds in recombinant human EGF is identical to that of mouse EGF. EGF is structurally homologous to human transforming growth factor-α (TGF-alpha), and both exert their actions through EGF receptors. EGF is homologous to a sequence contained in a 19 kD protein of vaccinia virus, which appears to utilize the EGF receptor to gain entry into cells.

EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells. 
Other Notes The biological activity of recombinant, human EGF is measured by its ability to stimulate the mouse fibroblast cell line, BALB/3T3. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay. 
性质
recombinant expressed in Escherichia coli 
assay ≥97% (SDS-PAGE) 
form lyophilized powder 
potency 0.08-0.8 ng/mL ED50/EC50 
total impurities ≤1 EU/μg endotoxin(EGF) 
EQP level Premium 
suitability suitable for cell culture 
storage temp. 20°C 
Gene Information Research your gene in Your Favorite Gene powered by Ingenuity
human ... EGF(1950) 
Articles
1. Epidermal Growth Factors (EGF) and NRG Family
Jennifer Fries
Biofiles Volume 4 Article 5 
2. Fibroblast Growth Factor Family (FGF)
Jennifer Fries
Biofiles Volume 4 Article 5 
3. Growth Factors in Stem Cell Biology
Jennifer Fries
Biofiles Volume 4 Article 5 

4. Hematopoietic Cytokines
Jennifer Fries
Biofiles Volume 4 Article 5 

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安全性
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter 
WGK Germany 3