UBE2M polyclonal antibody
产品名称： UBE2M polyclonal antibody
英文名称： UBE2M polyclonal antibody
产品编号： PAB2237
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of UBE2M.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBE2M.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.
Gong L, Yeh ET.J Biol Chem. 1999 Apr 23;274(17):12036-42.
- 2.
- A new NEDD8-ligating system for cullin-4A.
Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S.Genes Dev. 1998 Aug 1;12(15):2263-8.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of UBE2M polyclonal antibody (Cat # PAB2237) in Y-79 cell line lysates (35 ug/lane). UBE2M (arrow) was detected using the purified polyclonal antibody.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the UBE2M polyclonal antibody (Cat # PAB2237) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 9040
- Protein Accession#:
- NP_003960
- Gene Name:
- UBE2M
- Gene Alias:
- UBC-RS2,UBC12,hUbc12
- Gene Description:
- ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
- Omim ID:
- 603173
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq
- Other Designations:
- nedd8-conjugating enzyme Ubc12,ubiquitin carrier protein M,ubiquitin-conjugating enzyme E2M,ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12),ubiquitin-protein ligase M,yeast UBC12 homolog
- Gene Pathway