细胞周期素B2(CCNB2)重组蛋白

YB265Hu012
Recombinant Cyclin B2 (CCNB2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
细胞周期素B2(CCNB2)重组蛋白[ PROPERTIES ]
Residues: Val58~Ser398
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O95067
Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
细胞周期素B2(CCNB2)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.1
Predicted Molecular Mass: 42.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
细胞周期素B2(CCNB2)重组蛋白than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
VPV QPTKTTNVNK QLKPTASVKP VQMEKLAPKG PSPTPEDVSM KEENLCQAFS
DALLCKIEDI DNEDWENPQL CSDYVKDIYQ YLRQLEVLQS INPHFLDGRD INGRMRAILV
DWLVQVHSKF RLLQETLYMC VGIMDRFLQV QPVSRKKLQL VGITALLLAS KYEEMFSPNI
EDFVYITDNA YTSSQIREME TLILKELKFE LGRPLPLHFL RRASKAGEVD VEQHTLAKYL
MELTLIDYDM VHYHPSKVAAAASCLSQKVL GQGKWNLKQQ YYTGYTENEV LEVMQHMAKN
VVKVNENLTK FIAIKNKYAS SKLLKISMIP QLNSKAVKDL ASPLIGRS