小核糖核蛋白多肽C(SNKL)重组蛋白

YB374Hu016
Recombinant Small Nuclear Ribonucleoprotein Polypeptide C (SNRPC)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 小核糖核蛋白多肽C(SNKL)重组蛋白[ PROPERTIES ]
Residues: Pro2~Arg159
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P09234
Host: E. coli
Subcellular Location: Nucleus.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 小核糖核蛋白多肽C(SNKL)重组蛋白Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 9.8
Predicted Molecular Mass: 21.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8
oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 小核糖核蛋白多肽C(SNKL)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
PKFYCDYCD TYLTHDSPSV RKTHCSGRKH KENVKDYYQK WMEEQAQSLI DKTTAAFQQG
KIPPTPFSAP PPAGAMIPPP PSLPGPPRPG MMPAPHMGGP PMMPMMGPPP
PGMMPVGPAP GMRPPMGGHM PMMPGPPMMR PPARPMMVPT RPGMTRPDR
[ REFERENCES ]
1. Sillekens P.T.G., et al. (1988) Nucleic Acids Res. 16:8307-8321.
2. Yamamoto K., et al. (1988) J. Immunol. 140:311-317.
3. Heinrichs V., et al. (1990) Science 247:69-72.
4. Nelissen R.L.H., et al. (1991) Nucleic Acids Res. 19:449-454.