NUP50 polyclonal antibody
产品名称： NUP50 polyclonal antibody
英文名称： NUP50 polyclonal antibody
产品编号： PAB3708
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of NUP50.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human NUP50.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Nucleocytoplasmic transport: more than the usual suspects.
Swaminathan S, Melchior F.Dev Cell. 2002 Sep;3(3):304-6.
- 2.
- Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import.
Lindsay ME, Plafker K, Smith AE, Clurman BE, Macara IG.Cell. 2002 Aug 9;110(3):349-60.
- 3.
- Characterization and targeted disruption of murine Nup50, a p27(Kip1)-interacting component of the nuclear pore complex.
Smitherman M, Lee K, Swanger J, Kapur R, Clurman BE.Mol Cell Biol. 2000 Aug;20(15):5631-42.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis of NUP50 polyclonal antibody (Cat # PAB3708). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human brainreacted with NUP50 polyclonal antibody (Cat # PAB3708), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 10762
- Protein Accession#:
- NP_705931;Q8N6V5
- Gene Name:
- NUP50
- Gene Alias:
- MGC39961,NPAP60,NPAP60L
- Gene Description:
- nucleoporin 50kDa
- Omim ID:
- 604646
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000028685,nuclear pore-associated protein 60L
- Related Disease