PLD1 polyclonal antibody
产品名称： PLD1 polyclonal antibody
英文名称： PLD1 polyclonal antibody
产品编号： PAB2009
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PLD1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PLD1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Phospholipase D1 is an effector of Rheb in the mTOR pathway.
Sun Y, Fang Y, Yoon MS, Zhang C, Roccio M, Zwartkruis FJ, Armstrong M, Brown HA, Chen J.Proc Natl Acad Sci U S A. 2008 Jun 17;105(24):8286-91. Epub 2008 Jun 11.
- 2.
- Three adjacent binding sites for cAMP receptor protein are involved in the activation of the divergent malEp-malKp promoters.
Vidal-Ingigliardi D, Raibaud O.Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):229-33.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of PLD1 polyclonal antibody (Cat # PAB2009) in HeLa cell line lysates (35 ug/lane). PLD1 (arrow) was detected using the purified Polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human testis tissue reacted with PLD1 polyclonal antibody (Cat # PAB2009) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 5337
- Protein Accession#:
- NP_002653;Q13393
- Gene Name:
- PLD1
- Gene Alias:
- -
- Gene Description:
- phospholipase D1, phosphatidylcholine-specific
- Omim ID:
- 602382
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis (Hammond et al., 1995 [PubMed 8530346]).[supplied by OMIM
- Other Designations:
- choline phosphatase 1,phospholipase D1, phophatidylcholine-specific