AKR1B1 polyclonal antibody
产品名称： AKR1B1 polyclonal antibody
英文名称： AKR1B1 polyclonal antibody
产品编号： PAB2582
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of AKR1B1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human AKR1B1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Upregulation of aldose reductase during foam cell formation as possible link among diabetes, hyperlipidemia, and atherosclerosis.
Gleissner CA, Sanders JM, Nadler J, Ley K.Arterioscler Thromb Vasc Biol. 2008 Jun;28(6):1137-43. Epub 2008 May 1.
- 2.
- Merging the binding sites of aldose and aldehyde reductase for detection of inhibitor selectivity-determining features.
Steuber H, Heine A, Podjarny A, Klebe G.J Mol Biol. 2008 Jun 20;379(5):991-1016. Epub 2008 Apr 8.
- 3.
- Cloning and prokaryotic expression of a biologically active human placental aldose reductase.
Grundmann U, Bohn H, Obermeier R, Amann E.DNA Cell Biol. 1990 Apr;9(3):149-57.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of AKR1B1 polyclonal antibody (Cat # PAB2582) in Jurkat cell line lysates (35 ug/lane).AKR1B1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human colon carcinomareacted with AKR1B1 polyclonal antibody (Cat # PAB2582), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 231
- Protein Accession#:
- NP_001619;P15121
- Gene Name:
- AKR1B1
- Gene Alias:
- ADR,ALDR1,ALR2,AR,MGC1804
- Gene Description:
- aldo-keto reductase family 1, member B1 (aldose reductase)
- Omim ID:
- 103880
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq
- Other Designations:
- Lii5-2 CTCL tumor antigen,aldehyde reductase 1,aldo-keto reductase family 1, member B1,aldose reductase,low Km aldose reductase
- Related Disease
- Albuminuria
- Cardiovascular Diseases
- Coronary Disease
- Diabetes Complications
- Diabetes Mellitus, Type 1
- Diabetes Mellitus, Type 2
- Diabetic Angiopathies
- Diabetic Nephropathies
- Diabetic Neuropathies
- Diabetic Retinopathy
- Disease Progression
- Edema
- Genetic Predisposition to Disease
- Glomerulonephritis, IGA
- Hypospadias
- Kidney Failure, Chronic
- Renal Insufficiency, Chronic