Hairless,无毛发蛋白抗体

产品编号HZ-8507R
英文名称Hairless
中文名称无毛发蛋白抗体
别 名HR; ALUNC; AU; HAIR_HUMAN; Hairless protein; Host range; HSA277165; Protein hairless.
说 明 书0.1ml 0.2ml
研究领域染色质和核信号 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
Hairless,无毛发蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量127kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Hairless
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Hairless,无毛发蛋白抗体PubMedPubMed
产品介绍background:
Hairless is a 1,189 amino acid protein which is expressed as two isoforms produced by alternative splicing. The two isoforms are expressed in a variety of tissues in varying concentrations. Isoform 1 is more abundant than isoform 2 and is expressed at low levels in kidneys and testis, while isoform 2 is expressed abundantly in skin. Both isoforms are also present together in many tissues and are expressed strongly in small intestine and brain and weakly in trachea. HR is thought to be a transcription factor involved in hair growth. Hair growth occurs in three phases known as anagen, catagen and telogen, which are phases where growth, regression and rest, respectively, are taking place. By unknown mechanisms, HR is thought to regulate one of the hair growth phases and to work with vitamin D receptor (VDR) to regulate hair follicle cycling. Defects in HR may cause two serious ailments, known as alopecia universalis congenita (ALUNC) and atrichia with papular lesions (APL), which is also referred to as congenital atrichia. Both are autosomally recessive impairments. ALUNC is a rare condition in which hair follicles are produced without hair, while APL is a serious disease in which papillary lesions may cover the body and little to no hair is grown.

Function:
May act as a transcription factor that could act on to regulate one of the phases of hair growth.

Hairless,无毛发蛋白抗体Subcellular Location:
Nucleus.

Tissue Specificity:
Strongest expression of isoforms 1 and 2 is seen in the small intestine, weaker expression in brain and colon, and trace expression is found in liver, pancreas, spleen, thymus, stomach, salivary gland, appendix and trachea. Isoform 1 is always the most abundant. Isoform 1 is exclusively expressed at low levels in kidney and testis. Isoform 2 is exclusively expressed at high levels in the skin.

DISEASE:
Defects in HR are the cause of alopecia universalis congenita (ALUNC) [MIM:203655]. ALUNC is a rare autosomal recessive form of hair loss characterized by hair follicles without hair.
Defects in HR are the cause of atrichia with popular lesions (APL) [MIM:209500]; also known as congenital atrichia. APL is an autosomal recessive disease characterized by papillary lesions over most of the body and almost complete absence of hair.
Defects in HR are the cause of hypotrichosis type 4 (HYPT4) [MIM:146550]. An autosomal dominant condition characterized by reduced amount of hair, alopecia, little or no eyebrows, eyelashes or body hair, and coarse, wiry, twisted hair in early childhood.

Similarity:
Contains 1 JmjC domain.

Database links:

UniProtKB/Swiss-Prot: O43593.5

Hairless,无毛发蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Hairless Polyclonal Antibody, Unconjugated(HZ-8507R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Hairless Polyclonal Antibody, Unconjugated(HZ-8507R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining