重组人SIRP α蛋白（Recombinant Human SIRPα Protein），GMP级

产品货号：GMP-TL729

产品描述（Description）

Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells。

分子别名（Synonym）

SHPS1、SIRPA、CD172A、BIT、MFR、MYD1、P84、PTPNS1

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人SIRP alpha（NCBI Reference Sequence:NP_542970.1）表达带有His标签在C末端

分子量（Molecular Weight）

重组人SIRP alpha蛋白 包含241个氨基酸，预测理论分子量为39kD

纯度（Purity）

≥95%采用 SDS-PAGE 凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 CD47(Fc 标签)5ug/mL(100 ul/孔)能够结合人 SIRPα(His 标签)的线性范围是 10-500 ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

产品关键词：重组人SIRPα蛋白、SIRPα;

参考文献

1. Signal regulatory protein alpha (SIRPα) regulates the homeostasis of CD103+CD11b+ DCs in the intestinal lamina propria Charlotte L Scott, Zangerle Murray TFP, Katherine S H Beckham, Gillian Douce, Allan McI Mowat Eur J Immunol. 2014 Dec; 44(12): 3658–3668. Published online 2014 Oct 27. doi: 10.1002/eji.201444859

  注意：For research and manufacturing use.