PRKAB1 polyclonal antibody
产品名称： PRKAB1 polyclonal antibody
英文名称： PRKAB1 polyclonal antibody
产品编号： PAB4575
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PRKAB1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PRKAB1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus.
Minokoshi Y, Alquier T, Furukawa N, Kim YB, Lee A, Xue B, Mu J, Foufelle F, Ferre P, Birnbaum MJ, Stuck BJ, Kahn BB.Nature. 2004 Apr 1;428(6982):569-74.
- 2.
- AMP-activated protein kinase plays a role in the control of food intake.
Andersson U, Filipsson K, Abbott CR, Woods A, Smith K, Bloom SR, Carling D, Small CJ.J Biol Chem. 2004 Mar 26;279(13):12005-8. Epub 2004 Jan 23.
- 3.
- C75, a fatty acid synthase inhibitor, modulates AMP-activated protein kinase to alter neuronal energy metabolism.
Landree LE, Hanlon AL, Strong DW, Rumbaugh G, Miller IM, Thupari JN, Connolly EC, Huganir RL, Richardson C, Witters LA, Kuhajda FP, Ronnett GV.J Biol Chem. 2004 Jan 30;279(5):3817-27. Epub 2003 Nov 13.
- Applications
- Western Blot (Tissue lysate)
- The PRKAB1 polyclonal antibody (Cat # PAB4575) is used in Western blot to detect PRKAB1 in mouse spleen tissue lysate .
- Western Blot (Cell lysate)
- The PRKAB1 polyclonal antibody (Cat # PAB4575) is used in Western blot to detect PRKAB1 in Jurkat cell lysate .
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PRKAB1 polyclonal antibody (Cat # PAB4575) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Entrez GeneID:
- 5564
- Protein Accession#:
- AAKB_HUMAN
- Gene Name:
- PRKAB1
- Gene Alias:
- AMPK,HAMPKb,MGC17785
- Gene Description:
- protein kinase, AMP-activated, beta 1 non-catalytic subunit
- Omim ID:
- 602740
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq
- Other Designations:
- 5'-AMP-activated protein kinase beta-1 subunit,AMP-activated protein kinase beta 1 non-catalytic subunit,AMP-activated protein kinase beta subunit,AMPK beta -1 chain,AMPK beta 1,protein kinase, AMP-activated, noncatalytic, beta-1