透明带糖蛋白2(ZP2)重组蛋白

YB612Ra011
Recombinant Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
透明带糖蛋白2(ZP2)重组蛋白[ PROPERTIES ]
Residues: Leu353~Pro616
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O54767
Host: E. coli
Subcellular Location: Cell membrane,
Extracellular matrix. Membrane, Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
透明带糖蛋白2(ZP2)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.2
Predicted Molecular Mass: 33.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
透明带糖蛋白2(ZP2)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
LCTRDGFM DFEVYSHQTK PALNLESLLV GNSSCQPIFK VQSLGLARFH IPLNGCGTRQ
KFEGDKVIYE NEIHALWENP PSNIIFRNSE FRMTVRCHYI RDSMLLRAHI KSHSSPVASV
KPGPLALVLQ TYPDISYQRP YRKNEYPLVR YLRQPIYMEV TVLNRNDPNI KLVLDDCWAT
TFEDPASVPQ WQIIMDGCEY ELDNYRTTFH AANSSAAHSG HYQRFDVKTF AFVSESRGLS
SLIYFHCSAL ICNQASPLCS VTCPAP