FITC标记的高分子量神经丝蛋白抗体
产品名称： FITC标记的高分子量神经丝蛋白抗体
英文名称： Anti-NF-H/FITC
产品编号： HZ-0708R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： Flow-Cyt=1:50-200 IF=1:50-200
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
- 二维码 : 点击查看
Rabbit Anti-NF-H/FITC Conjugated antibody
FITC标记的高分子量神经丝蛋白抗体
| 英文名称 | Anti-NF-H/FITC |
| 中文名称 | FITC标记的高分子量神经丝蛋白抗体 |
| 别 名 | Neurofilament 200; 200 kDa neurofilament protein; 200 kD Neurofilament Heavy; NEFH; NEFH; NF200; NF-200; Neurofilament H; Neurofilament heavy polypeptide 200kD; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; Neurofilament triplet H protein; Hypophosphorylated Neurofilament H; NF H; NFH; NFH_HUMAN; KIAA0845. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | Flow-Cyt=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 118kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NF-H |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia. Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Post-translational modifications: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. DISEASE: Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. Similarity: Belongs to the intermediate filament family. Database links: Entrez Gene: 4744 Human Entrez Gene: 380684 Mouse Entrez Gene: 24587 Rat Omim: 162230 Human SwissProt: P12036 Human SwissProt: P19246 Mouse SwissProt: P16884 Rat Unigene: 198760 Human Unigene: 298283 Mouse Unigene: 108194 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
神经丝可以被定义为特别是在神经元细胞中发现的中间或10nm长丝。当用电子显微镜观察时，神经丝表现为直径为10纳米、长度不确定的纤维，其两侧通常有细小的细长突起。它们在大投射神经元轴突中特别丰富。它们可能起到为神经元及其突触提供结构支持以及支持快速传导轴突下冲动所需的大轴突直径的作用。神经丝是由亚基组成的混合物，通常包括三种神经丝三重体蛋白：神经丝轻（NFL）、神经丝介质（NFM）和神经丝重（NFH）。神经丝也可包括少量的外周素、α内生长素、巢蛋白和一些情况下波形蛋白。各种神经丝亚单位的抗体是非常有用的细胞类型标记，因为蛋白质是神经系统中最丰富的一种，只在神经元中表达，并且在生化上非常稳定。一些研究表明，阿尔茨海默病、额颞叶痴呆和血管性痴呆患者的神经丝重量和神经丝光的水平升高。