重组人PD-1蛋白(Fc)|Recombinant Human PD-1 Protein(Fc)，GMP级

产品货号：GMP-TL732

产品描述（Description）

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

分子别名（Synonym）

PDCD1、CD279、SLEB2

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人PD-1（NCBI Reference Sequence: NP_005009.2）表达带有Fc标签在C末端

分子量（Molecular Weight）

重组人PD-1蛋白包含380个氨基酸，预测理论分子量为43.4kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 PD-L1(His 标签）的浓度是 5μg/mL(100uL/孔) 能够结合人 PD-1(Fc 标签)的线性范围是 0.0625-1ug/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH7.4）的冻干粉

参考文献

1. PD-1 Expression on Mycobacterium tuberculosis-Specific CD4 T Cells Is Associated With Bacterial Load in Human Tuberculosis Cheryl L. Day, Deborah A. Abrahams, Rubina Bunjun, Lynnett Stone, Marwou de Kock, Gerhard Walzl, Robert J. Wilkinson, Wendy A. Burgers, Willem A. Hanekom Front Immunol. 2018; 9: 1995. Published online 2018 Aug 31. doi: 10.3389/fimmu.2018.01995

  注意：For research and manufacturing use.