NARF,核纤层蛋白A识别因子抗体

产品编号HZ-11173R
英文名称NARF
中文名称核纤层蛋白A识别因子抗体
别 名DKFZp434G0420; FLJ10067; GC17P078009; IOP2; Iron only hydrogenase like protein 2; Nuclear prelamin A recognition factor; Prenyl dependent prelamin A binding NARF_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 细胞骨架 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
NARF,核纤层蛋白A识别因子抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量51kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human NARF
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
NARF,核纤层蛋白A识别因子抗体PubMedPubMed
产品介绍background:
Prenylation and methylation are two forms of protein modification, both of which are important for a variety of functions, including membrane attachment, protein-protein interactions and signaling events. NARF (nuclear prelamin A recognition factor), also known as IOP2, is a 456 amino acid nuclear protein that belongs to the NARF family. Expressed ubiquitously with highest expression in heart, skeletal muscle and brain, NARF binds to the C-terminal end of prenylated prelamin A and may be a member of a prelamin A-containing endoprotease complex. Additionally, via its association with prelamin A, NARF may be involved in heterochromatin organization. NARF is expressed as three isoforms due to alternative splicing events and, upon DNA damage, may be phosphorylated by ATM or ATR.

Function:
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

Subunit:
Interacts with LMNA and binds to the farnesylated C-terminal domain.

Subcellular Location:
Nuclear

NARF,核纤层蛋白A识别因子抗体Tissue Specificity:
Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the NARF family.

Gene ID:
26502

Database links:
Entrez Gene: 618340 Cow
Entrez Gene: 26502 Human
Entrez Gene: 67608 Mouse
Entrez Gene: 360681 Rat
Omim: 605349 Human
SwissProt: Q9UHQ1 Human
SwissProt: Q9CYQ7 Mouse
SwissProt: Q2YDU6 Rat
Unigene: 256526 Human
Unigene: 291832 Mouse
Unigene: 162225 Rat

NARF,核纤层蛋白A识别因子抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片	Sample: Muscle (Mouse)Lysate at 40 ug Heart (Mouse)Lysate at 40 ug Primary: Anti-NARF(HZ-11173R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 51kD Observed band size:51kD