3D cell culture the easy way
Scaffold-free re-aggregation of single cells into functional 3D microtissues has been a tricky task in the past. With the introduction of the GravityPLUS?platform in 2009, InSphero made reliable, automation-compatible and affordable 3D cell culture in hanging drops a reality. Since its introduction, the GravityPLUS?platform has proven its ease of use and versatility 1000s of times in laboratories ranging from academic research to high-throughput screening.
Today, all top 15 global pharmaceutical and cosmetics companies use InSphero's 3D microtissues produced using the GravityPLUS?platform. Contact us today to try it, too!
Two plates, one platform
The patented GravityPLUS?plate makes hanging-drop cell culture easy. Simply load up to 50 霯 cell suspension from the top with any liquid handling device ?from a manual pipette to a 96-tip robot. Within 2-4 days, a single microtissue forms in each drop. For long-term culture or differentiation, you can even remove up to 70% of the medium and replace it through the top port.
Once the microtissues are formed, they can easily be harvested into the GravityTRAP?plate with a simple media addition step. The proprietary non-adhesive coating of the GravityTRAP?plate allows for culturing over weeks without attachment and decomposition of the microtissues. In addition, its unique design allows reliable medium exchange without microtissue aspiration and excellent imaging, e.g. high-content analysis, through a window at the well bottom.
Precise size control: SureDrop?/H2>
A reliable 3D cell-based assay starts with precise control over the microtissue size. InSphero's products are the only on the market featuring our patent-pending SureDrop?microtechnology, which allows for precise dispensing into and aspirating from hanging drops. As the drop volume corresponds to the seeding cell number, microtissues produced in GravityPLUS?plates display outstandingly low diameter variations down to 5% and less.
The SureDrop?wells are elastically suspended and mechanically adjust to the pipette tip to assure a tight seal between tip and inlet. SureDrop?eliminates operator and instrument variations reliably.
High-content image of a HepG2 microtissue produced in the GravityPLUS?platform. Image provided by Imagen Ltd. (www.imagen-biotech.com)
