7-AAD Cell Viability Assay Kit-蛋白检测-试剂-生物在线

产品名称： 7-AAD Cell Viability Assay Kit

英文名称： 7-AAD Cell Viability Assay Kit

产品编号： KA1353

产品价格： 0

产品产地：台湾

品牌商标： Abnova

更新时间： null

使用范围： null

亚诺法生技股份有限公司（Abnova)

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Product Description:
7-AAD Cell Viability Assay Kit is a fluorescence-labeled method for screening dead or damaged cells.

Flow Cytometry
Effects of H₂O₂ on cell viability measured by 7-AAD. THP-1 cells were seeded in a 6-well plate at a density of 10⁶ cells/well and cultured in a CO₂ incubator overnight. The next day, cells were treated with vehicle (upper and lower left panels), 0.05% (15 mM) H₂O₂ (upper and lower middle panels), or 0.25% (75 mM) H₂O₂ (upper and lower right panels) for 24 hours at 37°C in a CO₂ incubator. Cells were then pelleted and stained with Staining Solution according to the procedure described in this booklet. In the cells treated with vehicle (control), there were few dead cells (1.3%, lower right quadrant of lower left panel). H₂O₂ at 0.05% caused death of 36.5% of the cells in a 24 hour period (lower right quadrant of lower middle panel). When cells were treated with 0.25% H₂O₂ for 24 hours, most of cells were dead (82.5%, lower right quadrant of l